RELATIONSHIP OF CLINICAL AND MORPHOLOGICAL PARAMETERS WITH SURVIVAL IN PATIENTS WITH ADENOCARCINOMA OF THE LUNG WITH ACTIVE NUCLEOLAR ORGANIZERS IN THE MIB-1 POSITIVE CELLS
https://doi.org/10.18027/2224-5057-2014-3-57-59
Abstract
Aim: To investigate argyrophilic proteins associated with nucleolar organizer regions (Ag-NOR proteins) in proliferating cells in connection with clinical and morphological parameters and survival in patients with adenocarcinoma of the lung.
Materials and methods: Specimens of 94 operated patients with lung adenocarcinoma were investigated using double staining for antigen Ki-67 (Clone MIB-1, DAKO) by immunohistochemistry and Ag-NOR proteins staining with silver nitrate.
Results: In the tumor tissue Ag-NOR proteins in the MIB-1 positive cells correlated with clinical and morphological parameters on TNM-system: T-index, the tumor size, N-index, disease stage and differentiation. Survival of patients with a small area of Ag-NOR proteins in the MIB-1 positive cells was better than in patients with large area. The area of Ag-NOR proteins in the MIB-1 positive cells – is an independent prognostic factor in patients with adenocarcinoma of the lung.
Conclusion: The area of Ag-NOR proteins in the MIB-1 positive cells correlated with clinical and morphological parameters of the TNM-system and survival in patients with adenocarcinoma of the lung.About the Authors
Dmitriy S. Kobyakov
Когалымская городская больница
Russian Federation
Russian Federation
Ashot M Avdalyan
Российский онкологический научный центр им. Н. Н. Блохина, Алтайский филиал
Russian Federation
Russian Federation
Aleksandr F. Lazarev
Российский онкологический научный центр им. Н. Н. Блохина, Алтайский филиал
Russian Federation
Russian Federation
Vladimir V. Klimatchev
Алтайский государственный медицинский университет, Барнаул
Russian Federation
Russian Federation
Igor P. Bobrov
Алтайский государственный медицинский университет, Барнаул
Russian Federation
Russian Federation
References
1. Abboud P., Lorenzato M., Joly D., Quereux C., Birembaut P., Ploton D. Prognostic value of a proliferation index including MIB1 and argyrophilic nucleolar organizer regions proteins in node-negative breast cancer. Am J Obstet Gynecol. 2008 199 (2):146.e1–7.
2. Biesterfeld S., Farokhzad F., Klüppel D., Schneider S., Hufnagl P. Improvement of breast cancer prognostication using cell kinetic-based silver-stainable nucleolar organizer region quantification of the MIB-1 positive tumor cell compartment. Virchows Arch. 2001 438 (5):478–84.
3. Bigras G., Marcelpoil R., Brambilla E., Brugal G. Interest of targeting AgNORs measurement in cycling cells: in vivo cell kinetic evaluation of non-small cell lung cancer. Anal Cell Pathol. 1996 11 (3):183–98.
4. Canet V., Montmasson M. P., Usson Y., Giroud F., Brugal G. Correlation between silver-stained nucleolar organizer region area and cell cycle time. Cytometry. 2001 43 (2):110–6.
5. Kidogawa H., Nanashima A., Yano H., Matsumoto M., Yasutake T., Nagayasu T. Clinical significance of double staining of MIB-1 and AgNORs in primary breast carcinoma. Anticancer Res. 2005 25 (6B):3957–62.
6. Lorenzato M., Abboud P., Lechki C., Browarnyj F., O’Donohue M.F., Ploton D., Adnet J. J. Proliferation assessment in breast cancer: a double-staining technique for AgNOR quantification in MIB-1 positive cells especially adapted for image cytometry. Micron. 2000 31 (2):151–9.
7. Munakata S., Hendricks J. B. A multilabeling technique for simultaneous demonstration and quantitation of Ki-67 and nucleolar organizer regions (AgNORs) in paraffin-embedded tissue. J Histochem Cytochem. 1994 42 (6):789–3.
8. Ogura S., Abe S., Sukoh N., Kunikane H., Nakajima I., Inoue K., Kawakami Y. Correlation between nucleolar organizer regions visualized by silver staining and the growth rate of lung adenocarcinoma. Cancer. 1992 70 (1):63–8.
9. Pich A., Chiusa L., Margaria E. Prognostic relevance of AgNORs in tumor pathology. Micron. 2000 31 (2):133–41.
10. Scholzen T., Gerdes J. The Ki-67 protein: from the known and the unknown. J Cell Physiol. 2000 182 (3):311–22.
11. Sirri V, Roussel P, Hernandez-Verdun D. The AgNOR proteins: qualitative and quantitative changes during the cell cycle. Micron. 2000 31 (2):121–6.
12. Sobin L., Gospodarowicz M., Wittekind C. (Eds.): TNM classification of malignant tumours. 7th edition. Oxford, Wiley-Blackwell, 2009. Р. 138–46.
13. Tomobe M., Shimazui T., Uchida K., Hinotsu S., Akaza H. Argyrophilic nucleolar organizer region in proliferating cell has a predictive value for local recurrence in superficial bladder tumor. J Urol. 1999 162 (1):63–8.
14. Trere D. AgNOR staining and quantification. Micron. 2000 31 (2):127–31.
15. Yamaguchi S. Relationship between the Responses to Simultaneous Double Staining for Ki-67 and AgNOR and the Clinicopathological Features of Non-Small Cell Pulmonary Carcinoma. Acta Med Nagasaki 1994 39 (4): 147–52.
Review
For citations:
Kobyakov D.S., Avdalyan A.M., Lazarev A.F., Klimatchev V.V., Bobrov I.P. RELATIONSHIP OF CLINICAL AND MORPHOLOGICAL PARAMETERS WITH SURVIVAL IN PATIENTS WITH ADENOCARCINOMA OF THE LUNG WITH ACTIVE NUCLEOLAR ORGANIZERS IN THE MIB-1 POSITIVE CELLS. Malignant tumours. 2014;(3):57-59. (In Russ.) https://doi.org/10.18027/2224-5057-2014-3-57-59
Views:
1307
Email this article 