The role of microRNA genes methylation in different molecular-biological subtypes of breast cancer
https://doi.org/10.18027/2224-5057-2018-8-1-5-11
Abstract
The methylation of CpG islands in the promoter regions of miRNA genes is an epigenetic modification that plays a decisive role in the breast cancer (BC) initiation and progression. The aim of the study was to investigate the frequency of 5 miRNA genes methylation (miRNA-9–1, miRNA-9–3, miRNA-34b/c, miRNA-193A, miRNA-129-2) in mammary epithelial neoplasms. Methylation-specific polymerase chain reaction (MS-PCR) was used to detect methylated genes. 62 patients took part in this study. It was found that the frequency of all 5 miRNAs genes methylation is significantly higher in tumor tissue than in the adjacent histologically unchanged mammary tissue. The authors also performed a correlation analysis and founded a relationship between the methylation rate of certain miRNA genes with some clinical and molecular characteristics of the tumor. This information on epigenetic disorders of BC complements the “molecular portrait” of the tumor and can be used to diagnose and assess the prognosis.
About the Authors
D. A. Ryabchicov
N. N. Blokhin Russian Cancer Research Center
Russian Federation
MD, PhD Med, Leading Researcher, Department of Breast Cancer Surgical Treatment
Russian Federation
MD, PhD Med, Leading Researcher, Department of Breast Cancer Surgical Treatment
I. A. Dudina
I. M. Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation
5th year student
Russian Federation
5th year student
I. K. Vorotnikov
N. N. Blokhin Russian Cancer Research Center
Russian Federation
MD, DSc Med, Professor, Head of the Department of Breast Cancer Surgical Treatment
Russian Federation
MD, DSc Med, Professor, Head of the Department of Breast Cancer Surgical Treatment
O. A. Talipov
N. N. Blokhin Russian Cancer Research Center
Russian Federation
post-graduate student
Russian Federation
post-graduate student
K. S. Titov
The Loginov Moscow Clinical Scientific Center;
Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
MD, DSc Med, Head of the Department of Skin and Soft Tissues Oncosurgery
Associate Professor of the Department of Oncology and Radiation Therapy
D. A. Denchik
N. N. Blokhin Russian Cancer Research Center
Russian Federation
MD, PhD Med, Researcher, Department of Breast Cancer Surgical Treatment
Russian Federation
MD, PhD Med, Researcher, Department of Breast Cancer Surgical Treatment
T. P. Kazubskaya
N. N. Blokhin Russian Cancer Research Center
Russian Federation
MD, DSc Med, Leading Research Assistant, Clinical Oncogenetics Laboratory
Russian Federation
MD, DSc Med, Leading Research Assistant, Clinical Oncogenetics Laboratory
A. M. Burdennyy
Institute of General Pathology and Pathophysiology
Russian Federation
MD, PhD Biol, Senior Researcher, Pathogenomics and Transcriptomics Laboratory
Russian Federation
MD, PhD Biol, Senior Researcher, Pathogenomics and Transcriptomics Laboratory
V. I. Loginov
Institute of General Pathology and Pathophysiology
Russian Federation
MD, PhD Biol, Leading Researcher, Pathogenomics and Transcriptomics Laboratory
Russian Federation
MD, PhD Biol, Leading Researcher, Pathogenomics and Transcriptomics Laboratory
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Review
For citations:
Ryabchicov D.A., Dudina I.A., Vorotnikov I.K., Talipov O.A., Titov K.S., Denchik D.A., Kazubskaya T.P., Burdennyy A.M., Loginov V.I. The role of microRNA genes methylation in different molecular-biological subtypes of breast cancer. Malignant tumours. 2018;8(1):5-11. (In Russ.) https://doi.org/10.18027/2224-5057-2018-8-1-5-11
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