tumorsMalignant tumoursMalignant tumours2224-50572587-6813Rosoncoweb10.18027/2224-5057-2013-3-3-9tumors-47Research ArticleФУНДАМЕНТАЛЬНАЯ ОНКОЛОГИЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАFUNDAMENTAL ONCOLOGY AND EXPERIMENTAL MEDICINEОПРЕДЕЛЕНИЕ ПЕРЕСТРОЕК ГЕНА ALK В СЕЛЕКТИРОВАННОЙ ПОПУЛЯЦИИ РОССИЙСКИХ БОЛЬНЫХ НЕМЕЛКОКЛЕТОЧНЫМ РАКОМ ЛЕГКОГОDetermination of rearrangements in ALK gene in selected russian population of patients with non-small cell lung cancerДемидоваИ. А.DemidovaI. A.ЦепенщиковаЕ. О.TsepenshchikovaE. O.БариновА. А.BarinovA. A.ГагаринИ. М.GagarinI. M.СавеловН. А.SavelovN. A.ГриневичВ. Н.GrinevichV. N.ТюляндинС. А.TyulyandinS. A.ГБУЗ МГОБ 62 ДЗМ, лаборатория молекулярной диагностикиRussian FederationГБУЗ МГОБ 62 ДЗМ, отделение патологической анатомииRussian FederationГБУ РОНЦ им. Н. Н. Блохина РАМН, отделение клинической фармакологии и химиотерапииRussian Federation2013200520150339Copyright © Демидова И.А., Цепенщикова Е.О., Баринов А.А., Гагарин И.М., Савелов Н.А., Гриневич В.Н., Тюляндин С.А., 20152015Демидова И.А., Цепенщикова Е.О., Баринов А.А., Гагарин И.М., Савелов Н.А., Гриневич В.Н., Тюляндин С.А.Demidova I.A., Tsepenshchikova E.O., Barinov A.A., Gagarin I.M., Savelov N.A., Grinevich V.N., Tyulyandin S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.malignanttumors.org/jour/article/view/47

Определение перестроек гена ALK у больных немелкоклеточным раком легкого (НМРЛ) предложено в качестве обязательной процедуры большинством современных международных рекомендаций по диагностике и лечению онкологических заболеваний. Целью исследования было проведение тестирования методом флюоресцентной гибридизации in situ (FISH) транслокаций с участием гена ALK у пациентов, направленных на тестирование мутаций гена EGFR в рамках Программы Общества онкологов-химиотерапевтов «Совершенствование молекулярно-генетической диагностики с целью повышения эффективности противоопухолевого лечения» www.cancergenome.ru. Успешное тестирование проведено 189 из 200 больных НМРЛ без мутаций гена EGFR. Выявлено 12 позитивных случаев (6,3 %). Показана зависимость частоты выявления реаранжировок гена ALK от гистологической формы опухоли, статуса курения и возраста пациента. Подтверждена эффективность использования метода FISH для широкого тестирования перестроек гена ALK в группе больных с железистыми гистологическими типами НМРЛ.

Determination of rearrangements in ALK gene in patients with non-small cell lung cancer (NSCLC), proposed as a mandatory procedure in most current international guidelines for the diagnosis and treatment of cancer. The aim of the study was to conduct a test by fluorescent hybridization in situ (FISH) of translocations involving ALK gene in patients referred for testing for EGFR gene mutations in the framework of the Society of oncologists-chemotherapeutists "Improvement of molecular genetic diagnosis in order to improve the efficiency of anticancer treatment» www.cancergenome.ru. Successful tests conducted in 189 out of 200 patients with NSCLC without mutations EGFR. There 12 positive tests (6.3%). The dependence of the frequency of detection of gene rearrangements of the ALK histological forms of cancer, smoking status and age of the patient was shown. The efficiency of wide FISH testing for ALK gene rearrangements in patients with glandular histological types of NSCLC was confirmed.

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The authors declare that there are no conflicts of interest present.